At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.

1. Origins of Metabolic Profiling
Arthur B. Robinson and Noah E. Robinson
2. Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism
Monique Piraud, Séverine Ruet, Sylvie Boyer, Cécile Acquaviva, Pascale Clerc-Renaud, David Cheillan, and Christine Vianey-Saban
3. Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry
David S. Millington and Robert D. Stevens
4. Analysis of Organic Acids and Acylglycines for the Diagnosis of Related Inborn Errors of Metabolism by GC- and HPLC-MS
Giancarlo la Marca and Cristiano Rizzo
5. HPLC Analysis of Purines and Pyrimidines for the Clinical Biochemical Diagnosis of Related Inborn Errors of Metabolism
Giuseppe Lazzarino, Angela Maria Amorini, Valentina Di Pietro, and Barbara Tavazzi
6. Bile Acid Analysis in Various Biological Samples Using Ultra Performance Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (LC/ESI-MS)
Masahito Hagio, Megumi Matsumoto, and Satoshi Ishizuka
7. Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrometry
Jan C. van Dam, Cor Ras, and Angela ten Pierick
8. Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry
Rajan S. Kombu, Henri Brunengraber, and Michelle A. Puchowicz
9. Quantification of Pentose Phosphate Pathway (PPP) Metabolites by Liquid Chromatography-Mass Spectrometry (LC-MS)
Amber Jannasch, Miroslav Sedlak, and Jiri Adamec
10. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling
Ian D. Wilson
11. Gas Chromatography-Mass Spectrometry (GC-MS)-Based Metabolomics
Antonia Garcia and Coral Barbas
12. The Use of Two Dimensional Gas Chromatography-Time-of-Flight-Mass Spectrometry (GCxGC-TOF-MS) for Metabolomic Analysis of Polar Metabolites
Kimberly Ralston-Hooper, Amber Jannash, Jiri Adamec, and Maria Sepúlveda
13. LC-MS-Based Metabolomics
Sunil Bajad and Vladimir Shulaev
14. Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)-Based Metabolomics
Philip Britz-McKibbin
15. Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues
Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, and Matej Oreši¿
16. Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)-Based Shotgun Lipidomics
Giorgis Isaac
17. Processing and Analysis of GC/LC-MS-Based Metabolomics Data
Elizabeth Want and Perrine Masson
18. Nuclear Magnetic Resonance (NMR)-Based Drug Metabolite Profiling
Eva M. Lenz
19. Nuclear Magnetic Resonance (NMR)-Based Metabolomics
Hector C. Keun and Toby J. Athersuch
20. Slow Magic Angle Sample Spinning: A Non- or Minimal-Invasive Method for High Resolution 1H Nuclear Magnetic Resonance (NMR) Metabolic Profiling
Jian Zhi Hu
21. Processing and Modeling of Nuclear Magnetic Resonance (NMR) Metabolic Profiles
Timothy M.D. Ebbels, John C. Lindon, and Muireann Coen