Part I. General

Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations
Alec W. M. Simpson

Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert

Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
Adriaan Nelemans

Part II. Specialist Measurement Systems

Confocal Microscopy: Theory and Applications for Cellular Signaling
Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars

Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration
Anthony J. Morgan and Andrew P. Thomas

Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer
Ian C. B. Marshall, Izzy Boyfield, and Shaun McNulty

Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
Ian C. B. Marshall, Davina E. Owen, and Shaun McNulty

Part III. Measurement of Ca2+ Channel Activity

Measurement of Ca2+ Entry Using 45Ca2+
Mercedes Villarroya, Manuela G. López, María F. Cano-Abad, and Antonio G. García

Measurement of [3H]PN200-110 and [125I]w-Conotoxin MVIIA Binding
Kazuyoshi Hirota and David G. Lambert

Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons
Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani

Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release From Intracellular Stores

Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells
Alison Skippen, Philip Swigart, and Shamshad Cockcroft

Measurement of Inositol(1,4,5)Triphosphate Using a Stereospecific Radioreceptor Mass Assay
Darren Smart

Measurement of Calcium Fluxes in Permeabilized Cells Using a45Ca2+ Uptake and Release Assay
Robert A. Wilcox

Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells
Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann

Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
Aldebaran M. Hofer

Part V. Specialist Measurement Techniques

Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
Hideo Kanaide

Measurement of Calcium and Movement in Heart Cells
Leong L. Ng and Paulene A. Quinn

Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations
Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch

Measurement of Cytosolic-Free Ca2+ in Plant Tissue
Martin R. McAinsh and Carl K.-Y. Ng

Part VI. Ca2+-Sensitive Targets

Assay and Purification of Calmodulin-Dependent Protein Kinase
Rajendra K. Sharma

Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
Rajendra K. Sharma

Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
Danuta Kosk-Kosicka

Index