Since the discovery of the gene for green fluorescent protein(GFP), derived from jellyfish, this protein that emits a green glowhas initiated a revolution in molecular biosciences. With thistool, it is now possible to visualize nearly any protein ofinterest in any cell or tissue of any species. Since thepublication of the first edition, there have been tremendouslysignificant technological advances, including development of newmutant variants. Proteins are now available in yellow and blue, andNovel Fluorescent Proteins (NFPs) have expanded their utility indeveloping biosensors, biological markers, and other biologicalapplications.
This updated, expanded new edition places emphasis on the rise ofNFPs, including new chapters on NFP properties with detailedprotocols, applications of GFPs and NFPs in industry research, andbiosensors. This book provides a solid theoretical framework, alongwith detailed, practical guidance on use of GFPs and NFPs withdiscussion of potential pitfalls. The expert contributors providereal examples in showing how to tailor GFP/NFP to specific systems,maximize expression, and enhance detection.