A detailed technical review of label-free biosensor techniques with worked examples.

1. Label-free biosensors: an introduction Brian T. Cunningham; 2. Experimental design Robert Karlsson; 3. Extracting affinity constants from biosensor binding responses Rebecca L. Rich and David G. Myszka; 4. Extracting kinetic rate constants from binding responses Rebecca L. Rich and David G. Myszka; 5. Sensor surfaces and receptor deposition Matthew A. Cooper; 6. Macromolecular interactions Francis Markey; 7. Interactions with membranes and membrane receptors Matthew A. Cooper; 8. Application of SPR technology to pharmaceutical relevant drug-receptor interactions Walter Huber; 9. High-throughput analysis of biomolecular interactions and cellular responses with resonant waveguide grating biosensors Ye Fang, Jack Fang, Elizabeth Tran, Xinying Xie, Michael Hallstrom, and Anthony G. Frutos; 10. ITC-derived binding constants Richard K. Brown, Michael Brandts, Ronan O'Brien, and William B. Peters; 11. Impedance technology applied to cell-based assays Ryan McGuinness and Ed Verdonk.