1. The Simplest Protocol for Rapid and Long-Term Culture of Primary Epidermal Keratinocytes from Human and Mouse

Filipa Pinto, Daisuke Suzuki, and Makoto Senoo

2. Dermal-Epidermal Separation by Heat

Liyan Jian, Yu Cao, and Ying Zou

3. Dermal-Epidermal Separation by Enzyme

Liyan Jian, Yu Cao, and Ying Zou

4. Dermal-Epidermal Separation by Chemical

Liyan Jian, Yu Cao, and Ying Zou

5. Isolation and Characterization of Extracellular Vesicles from Keratinocyte Cultures

Sebastian Sjöqvist, Aya Imafuku, Dhanu Ghupta, and Samir EL Andaloussi

6. Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential

Carmen Segrelles, Karla Santos-de-Frutos, Jesús M. Paramio, and Corina Lorz

7. Quantification of Melanosome Transfer Using Immunofluorescence Microscopy and Automated Image Analysis

Aishwarya Sridharan, John Lim S.Y., Graham D. Wright, and Leah A. Vardy

8. Contribution of Immunohistochemistry in Revealing S100A7/JAB1 Colocalization in Psoriatic Epidermal Keratinocyte

Mariagrazia Granata, Evangelia Skarmoutsou, Maria Clorinda Mazzarino, Massimo Libra, and Fabio D'Amico

9. Digital Quantification of Epidermal Protein Expression in Paraffin-Embedded Tissue Using Immunohistochemistry

Manuel Valdebran, Eric H. Kowalski, Diana Kneiber, Jing Li, Jeffrey Kim, and Kyle T. Amber

10. Keratinocyte Differentiation by Flow Cytometry

Natalia Sanz-Gómez, Ana Freije, and Alberto Gandarillas

11. In Silico and In Vitro Considerations of Keratinocyte Nuclear Receptor Protein Structural Order for Improving Experimental Analysis

Rambon Shamilov, Matthew J. Staid, and Brian J. Aneskievich

12. Genetic Modification of Human Primary Keratinoctes by Lentiviral Vectors

Ana Freije, Natalia Sanz-Gómez, and Alberto Gandarillas

13. Generation of Knockout Human Primary Keratinocytes by CRISPR/Cas9

Serena Grossi, Gabriele Fenini, Paulina Hennig, Michela Di Filippo, and Hans-Dietmar Beer

14. In Vitro Wound Healing Assays to Investigate Epidermal Migration

Kegan Main, Constantinos M. Mikelis, and Colleen L. Doçi

15. Iterative Three-Dimensional Epidermis Bioengineering and Xenografting to Assess Long-Term Regenerative Potential in Human Keratinocyte Precursor Cells

Nicolas O. Fortunel, Emmanuelle Bouissou-Cadio, Julien Coutier, and Michèle T. Martin

16. Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse

Nicole Diette, Igor Kogut, and Ganna Bilousova

17. Biotin Identification Proteomics in Three-Dimensional Organotypic Human Skin Cultures

Calvin J. Cable, Nihal Kaplan, Spiro Getsios, Paul M. Thomas, and Bethany E. Perez White

18. Information and Statistical Analysis Pipeline for High-Throughput RNA Sequencing Data

Shinji Nakaoka and Keita Matsuyama

19. Cell-Extracellular Matrix Adhesion Assay

Mehmet Varol

20. Methods for Analysis of Keratinocyte Migration

Jie Liu and Jiaping Zhang

21. Scratch Wound Healing Assay

Simona Martinotti and Elia Ranzato

22. Photoprotective Activity Assay toward Ultraviolet B in Human Keratinocytes

Mehmet Varol

23. Photodynamic Therapy Assay

Mehmet Varol

24. A Method to Prepare Claudin-Modulating Recombinant Proteins

Keisuke Tachibana and Masuo Kondoh

Parisa Goodarzi, Hamidreza Aghayan, Moloud Payab, Bagher Larijani, Sepideh Alavi-Moghadam, Masoumeh Sarvari, Hossein Adibi, Fatemeh Khatami, Najmeh Foroughi Heravani, Mahdieh Hadavandkhani, and Babak Arjmand

26. Ciliated Epithelial Cell Differentiation at Air-Liquid Interface Using Commercially Available Culture Media

Dani Do Hyang Lee, Alina Petris, Robert E. Hynds, and Christopher O'Callaghan