1. Analysis of Mitochondrial Dysfunction by Microplate Reader in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Disorders

Tatiana R. Rosenstock, Congxin Sun, Georgina Wynne Hughes, Katherine Winter, and Sovan Sarkar

2. Generation and Hematopoietic Differentiation of Mesenchymal Stromal/Stem Cell-Derived Induced Pluripotent Stem Cell Lines for Disease Modeling of Hematopoietic and Immunological Diseases

Gülen Güney-Esken and Fatima Aerts-Kaya

3. Establishment of Human Induced Pluripotent Stem Cells from Multiple Sclerosis Patients

Onur Can Begentas, Dilara Koc, and Erkan Kiris

4. Monitoring Axonal Degeneration in Human Pluripotent Stem Cell Models of Hereditary Spastic Paraplegias

Xue-Jun Li, Yongchao Mou, Conrad Milton, and Zhenyu Chen

5. Efficient Generation of Functional Hepatocytes from Human Induced Pluripotent Stem Cells for Disease Modeling and Disease Gene Discovery

Satish Kumar, Joanne E. Curran, Sarah Williams-Blangero, and John Blangero

6. Autophagy Dysfunction as a Phenotypic Readout in hiPSC-Derived Neuronal Cell Models of Neurodegenerative Diseases

Congxin Sun, Tatiana R. Rosenstock, Malkiel A. Cohen, and Sovan Sarkar

7. Derivation of Induced Pluripotent Stem Cell (iPSC) Lines from Patient-Specific Peripheral Blood Mononuclear Cells (PBMC) Using Episomal Vectors

Vijay Bhaskar Reddy Konala, Swapna Nandakumar, Harshini Surendran, and Rajarshi Pal

8. Differentiating Induced Pluripotent Stem Cells toward Mesenchymal Stem/Stromal Cells

Shennea S. McGarvey, Michael Ferreyros, Igor Kogut, and Ganna Bilousova

9. A High-Efficiency Method for the Production of Endothelial Cells from Human Induced Pluripotent Stem Cells

Maryna Pavlova, Shennea S. McGarvey, Ganna Bilousova, and Igor Kogut

10. Image-Based Quantitation of Kainic Acid-Induced Excitotoxicity as a Model of Neurodegeneration in Human iPSC-Derived Neurons

Jana Talbot, Sueanne Chear, Andrew Phipps, Alice Pébay, Alex W. Hewitt, James C. Vickers, Anna E. King, and Anthony L. Cook

11. Amyloid ß (Aß) ELISA of Human iPSC-Derived Neuronal Cultures

Hirotaka Watanabe, Rei Murakami, and Hideyuki Okano

12. Creating Cell Model 2.0 for Pathogenic Mitochondrial DNA Mutation: iPSC Approach for LHON Mutation

Pragya Singh, Tyler Bahr, Xiaoxu Zhao, Peiqing Hu, Marcel Daadi, TaoSheng Huang, and Yidong Bai

13. CRISPR Guide RNA Library Screens in Human Induced Pluripotent Stem Cells

Yan Zhou, Qiang Fu, Huijun Shi, and Guangqian Zhou

14. Generation and Encapsulation of Human iPSC-Derived Vascular Smooth Muscle Cells for Proangiogenic Therapy

Biraja C. Dash and Henry C. Hsia

15. Methods to Induce Small-Scale Differentiation of iPS Cells into Dopaminergic Neurons and to Detect Disease Phenotypes

Akihiro Yamaguchi, Kei-ichi Ishikawa, and Wado Akamatsu

16. Modeling Early Neural Crest Development via Induction from hiPSC-Derived Neural Plate Border-Like Cells

Diogo Andrade Nani, Gabriella Shih Ping Hsia, Maria Rita Passos-Bueno, and Gerson Shigeru Kobayashi

17. Generation of Human Induced Pluripotent Stem Cells from Renal Epithelial Cells

Miao Yu, Xinglong Han, Lingqun Ye, Wei Lei, and Shijun Hu

18. A Protocol for Stepwise Differentiation of Induced Pluripotent Stem Cells into Retinal Pigment Epithelium

19. Genome Editing of Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes to Model Genetic Ocular Diseases

Heran Getachew, Blanca Chinchilla, and Rosario Fernandez-Godino

20. Generation of Cardiomyocytes and Endothelial Cells from Human iPSCs by Chemical Modulation of Wnt Signaling

Arline Joachim, Shiqiao Ye, and Ming-Tao Zhao

21. Immunoassay for Quantitative Detection of Antibody Transcytosis across the Blood-Brain Barrier In Vitro

Caroline Sodja, Deborah Callaghan, Arsalan S. Haqqani, Danica B. Stanimirovic, Willard J. Costain, and Anna Jezierski

22. Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells

Shermaine Huiping Tay, Winanto, Zi Jian Khong, Yong Hui Koh, and Shi Yan Ng

23. CRISPR/Cas-Mediated Knock-In of Genetically Encoded Fluorescent Biosensors into the AAVS1 Locus of Human Induced Pluripotent Stem Cells

David Stellon, Minh Thuan Nguyen Tran, Jana Talbot, Sueanne Chear, Mohd Khairul Nizam Mohd Khalid, Alice Pébay, James C. Vickers, Anna E. King, Alex W. Hewitt, and Anthony L. Cook

24. Cancer Stem Cell Initiation by Tumor-Derived Extracellular Vesicles

Said M. Afify, Ghmkin Hassan, Ting Yan, Akimasa Seno, and Masaharu Seno

25. Genome Editing Using Cas9-gRNA Ribonucleoprotein in Human Pluripotent Stem Cells for Disease Modeling

Noelia Benetó, Daniel Grinberg, Lluïsa Vilageliu, and Isaac Canals