1. Using FPbase: The Fluorescent Protein Database

Talley J. Lambert

2. Selecting the Fluorescent Protein for In Vivo Imaging Experiments

Mayank Sharma

3. Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo

Marcela Díaz and Leonel Malacrida

4. Directed Evolution of Fluorescent Proteins in Bacteria

Sara Mattson, Geraldine N. Tran, and Erik A. Rodriguez

5. Fluorescent Proteins: Crystallization, Structural Determination, and Non-Natural Amino Acid Incorporation

Rochelle D. Ahmed, Husam Sabah Auhim, Harley L. Worthy, and D. Dafydd Jones

6. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization

Alina Remeeva, Anna Yudenko, Vera V. Nazarenko, Oleg Semenov, Anastasia Smolentseva, Andrey Bogorodskiy, Ivan Maslov, Valentin Borshchevskiy, and Ivan Gushchin

7. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R.M. Beinlich, Esther Knieps-Grünhagen, Valentin Gordeliy, Karl-Erich Jaeger, Santi Nonell, Ulrich Krauss, Thomas Gensch, and Thomas Drepper

8. CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP₁₁ Tag into the Genome of a Human Cell Line

Ryo Tamura and Daichi Kamiyama

9. Fluorescent Protein-Based Approaches for Sub-Cellular Protein Localization in Plants

Mayank Sharma, Ralf Bernd Klösgen, and Bationa Bennewitz

10. Growth Rates Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions

Kara L. Schneider, Lucas E. Reibenspies, Thomas Nyström, and Sviatlana Shashkova

11. DNA Visualization Using Fluorescent Proteins

Xuelin Jin, Y. Tehee Kim, and Kyubong Jo

12. Quantification of Intracellular Citrate Concentrations with Genetically Encoded Biosensors

Yufeng Zhao, Kei Takahashi-Yamashiro, Yi Shen, and Robert E. Campbell

13. Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out

Finja Bohle, Andreas J. Meyer, and Stefanie J. Mueller-Schuessele

14. Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae

Mikel Lavilla-Puerta and Beatrice Giuntoli

15. Osmium Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells

Isei Tanida, Junji Yamaguchi, Soichiro Kakuta, and Yasuo Uchiyama

16. Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins

Hebang Yao, Hongmin Cai, and Dianfan Li

17. Determining Protein-Protein Interaction with GFP-Trap Beads

Irem Yilmazer, Melanie R. Abt, Yuanyuan Liang, David Seung, Samuel C. Zeeman, and Mayank Sharma