Modern Approaches to Animal Cell Technology is a collection of papers presented at the 1987 joint European Society for Animal Cell Technology-OHOLO conference held in Tiberias, Israel. Contributors explore modern approaches to animal cell technology and discuss the construction of the animal cell substrate, the physiology of those cells in a bioreactor type of environment, and the ways in which different products can be made from animal cells in culture and tested.
This book is comprised of 59 chapters divided into nine sections and begins by outlining the history of issues and decisions that were made regarding the acceptability of various cell substrates, along with the use of continuous cell lines in biotechnology. The next chapter explores the feasibility, reproducibility, and the sensitivity of the dot-blot filter hybridization test to detect minute amounts of residual cellular DNA. The reader is then introduced to cells and cell lines such as monoclonal antibodies; cell growth and development; physiology of cells; and the use of bioreactors for culturing animal cells. Downstream unit processes, vaccines, immune system products, and toxicity testing with animal cells are also considered.
This monograph will be a valuable resource for animal-cell technologists, biotechnologists, and microbiologists.

1. The Liberation of Animal Cells: Psychology of Changing Attitudes2. Cellular DNA Biologicals: Significance and QuantitationSection 1 New Cells and Cell Lines 3. Human Monoclonal Antibodies - Their Potential, Problems and Prospects 4. Production of Cancer Specific Monoclonal aAtibodies with Human-Human Hybridomas and Their Serum-free, High density, Perfusion Culture 5. Establishment of a Human Hybridoma Secreting Monoclonal Antibodies toward Rabies Virus 6. The Production of Bovine and Ovine Monoclonal Antibodies to Gonadal Steroids: The Use of Interspecific Hybridomas 7. The Immortalization of Differentiated Mammalian Cells 8. Structural Basis of Growth Factor Receptor Mediated Cellular Signals (Abstract) 9. Growth Control of Normal Human B Lymphocytes 10. Anti-proliferative Effects and Phenotypic Alterations Induced by Methylurea Derivatives in B16 Mouse Melanoma 11. Comparative Analysis of Major Biological Aspects of Human Hepatoma in Culture 12. Mini-review: Essential Strategies for the Application of Herpes Simplex Virus as a Vector for the Expression of Foreign Genes 13. The Glutamine Synthetase Gene: A Novel Marker for Gene Amplification in Mammalian Cells (Abstract) 14. Recombinant Animal Cell Lines for Production of Glycoproteins 15. Foreign Gene Expression in Rat Pituitary GH3 Cells 16. Use of Transfected and Amplified Drosophila Heat Shock Promoter Construction for Inducible Production of Toxic Mouse c-myc Proteins in CHO CellsSection 2 Medium Development 17. The Use of Glutamine-free Media for the Growth of Three Cell Lines in Microcarrier Culture 18. The Attachment of MDCK Cells to Three Types of Microcarriers in Different Serum-free Media 19. Spreading, Growth and Function of Cells Attached to a Plasma Clot Interphase between Agarose and the AtmosphereSection 3 Physiological Investigations 20. Investigations of the Influence of Physical Environment on the Cultivation of Animal Cells 21. Shear Sensitivity of Three Hybridoma Cell Lines in Suspension Culture 22. NMR Metabolic Studies of Human Breast Cancer Cells 23. Optimization of Glass-sphere Immobilized Bed Cultures 24. Examination of a Simple Model for the Diffusion of Oxygen into Dense Masses of Animal Cells 25. Kinetics of Prourokinase Production by Mammalian Cells in Culture 26. Specific Kinetic Patterns of Production of Monoclonal Antibodies in Batch Cultures and Consequences for Fermentation Processes 27. A Model System to Study Gene Expression and Scale up of Microcarrier Cultures of Recombinant DNA Manipulated CellsSection 4 Bioreactor Developments 28. Membrane-Stirrer-Reactor for Bubble Free Aeration and Perfusion 29. Immobilization of Hybridomas in Packed-bed Bioreactors 30. Production of Biologicals by Animal Cells Immobilized in Polyurethane Foam Matrix 31. Comparison of Oxygenation Methods in a 39L Stirred Bioreactor 32. Development of Large-scale Mammalian Cell Perfusion Culture Systems 33. Microcarrier DORMACELL does not Detectably Alter Viral or Plasmid DNA upon Direct Exposure 34. Macroporous Microcarriers 35. Growth of Animal Cells on Microbeads III: Semi Continuous in Situ Estimation of Numbers 36. Evaluation of the Continuous Perfusion Culture System for the Production of Monoclonal Antibodies 37. Recent Advances in the Control of Monitoring of Bioreactors 38. Automated Process Control for Culturing Animal CellsSection 5 Downstream Unit Processes 39. Design Criteria for the Purification of Biopolymers for the Pharmaceutical Industry 40. Use of a Dynamic Filtration Method for Separation of Animal Cells 41. Continuous Culture of Murine Hybridomas with Integrated Recovery of Monoclonal AntibodiesSection 6 Vaccine Products 42. Improved Production of Marek's Disease Vaccine with the Multi-tube Technology 43.