In Vitro Methods in Cell-Mediated Immunity focuses on methods for approaching cell-mediated immune responses in vitro.
This book provides, in simplified in vitro systems, a basis for understanding the mechanism of the in vivo response and discusses useful and reliable in vitro tests for cell-mediated immune responses in humans, where in vivo testing is often not possible.
The topics discussed include factors and activities produced in vitro by lymphocytes; biological implications of in vitro phenomena; and requirements and prospects for improved methodology. The leucocyte migration technique for in vitro detection of cellular hypersensitivity in man; proliferation of human blood lymphocytes stimulated by antigen in vitro; and virus plaque assay for antigen-sensitive are also elaborated in this text.
This publication is a good reference for microbiologist and immunologists, including medical students researching on in vitro models for cell-mediated immune reactions.

ParticipantsContributorsPrefaceIntroductory NoteSection A Sessions I. MethodsforStudy of Mediators II. Factors and Activities Produced in Vitro by Lymphocytes III. Biological Implications of in Vitro Phenomena IV. Requirements and Prospects for Improved MethodologySection ? Methods Index of Assays and Species 1. The Assay of Inhibition of Macrophage Migration and the Production of Migration Inhibitory Factor (MIF) and Skin Reactive Factor (SRF) in the Guinea Pig 2. Assay for Inhibition of Macrophage Migration 3. The "Migration-on-Agar" Technique for Measurement of Lymphocyte-Macrophage Interactions Associated With Delayed Hypersensitivity 4. In Vitro Technique of the Cell Migration from the Spleen and/or Artificial Fragments 5. The Capillary Tube Migration Inhibition Technique Applied to Human Peripheral Lymphocytes Using the Guinea Pig Peritoneal Exudate as the Indicator Cell Population 6. Method for the Production of MIF by Human Blood Lymphocytes 7. The Leucocyte Migration Technique for in Vitro Detection of Cellular Hypersensitivity in Man 8. In Vitro Assay for Inhibition of Migration of Human Blood Leucocytes 9. The Capillary Technique for Measurement of Inhibition of Leucocyte Migration: An Assessment of Cell-Mediated Immunity 10. The Use of Cultured Lymphoid Cells as Target Cells for the Detection of Migration Inhibitory Factor 11. An m Vitro Test for Delayed Hypersensitivity: Macrophage Spreading Inhibition (M.S.I.) 12. The Technique for Observation of Migration and Aggregation of Human Blood Macrophages 13. The in Vitro Macrophage Aggregation Assay for Delayed Hypersensitivity 14. Lymphocyte Activation and Lymphotoxin Production 15. Determination of Lymphocyte-Mediated Cytotoxicity by Release of Radioactivity from 14C-Thymidine Labeled Cells 16. A Simplified Assay for the Cytotoxic Activity (Lymphotoxin) of Lymphocyte Culture Supernatants 17. Cr-Release from Chicken Erythrocytes: An Assay System for Measuring the Cytotoxic Activity of "Nonspecifically" Activated Lymphocytes In Vitro 18. In Vitro Assay of Target Cell Lysis by Sensitized Lymphocytes 19. The Technique of Clonal Inhibition: A Quantitative Assay for Human Lymphotoxin Activity 20. Production and Assay of a Lymphocyte Derived "Proliferation Inhibitory Factor" (PIF) 21. Coulter Counter Evaluation of the Death of Fibroblasts Mediated by Sensitized Lymphocytes in the Presence of Specific Antigen 22. Colony Inhibition and Cytotoxicity Assays 23. The Methodology of Microassay for Cell-Mediated Immunity (MCI) 24. The Demonstration of Lymphocyte Mitogenic Factor in the Guinea Pig 25. Preparation and Assay of Mouse Mitogenic Factors 26. The Preparation of a Lymphocyte Transforming Factor from Sensitive Lymphocytes Stimulated by Antigen 27. Lymphocyte Transformation: The Proliferation of Human Blood Lymphocytes Stimulated by Antigen In Vitro 28. An Assay of Lymphocyte Blastogenesis Based on Measurement of the Rate of Protein Synthesis 29. Leucocyte Culture Methodology: Production and Assay of Soluble Factors 30. Preparation and Assay of an Inhibitor of DNA Synthesis and a Nonspecific Mitogen Elaborated by Human Lymphoblasts 31. Assays for Chemotactic Factors 32. A Plaque Inhibition Assay for Human Interferon Employing Human Neonate Skin Fibroblast Monolayers and Bovine Vesicular Stomatitis Virus 33. Production and Assay of Human Lymphocyte Interferon Induced by Antigens 34. In Vitro Production and Assessment of Activity of Skin Reactive Factors Released by Lymphoid Cells and Associated with Cellular Immunity 35. The Preparation and Purification of Transfer Factor 36.