Part I: Genotoxicity Assays

1. Ames Test (Bacterial Reverse Mutation Test): Why, When, and How to Use

            Araceli Pillco and Eduardo de la Peña

2. The Ames II and Ames MPF Penta I Assay: A Liquid Microplate Format Modification of the Classic Ames Test

            Sini Flückiger-Isler and Markus Kamber

3. Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles

            Christophe Pagnout, Stéphane Jomini, and Pascale Bauda

4. The Control of Hydrophobic Compound Exposure in In Vitro Tests for Genotoxicity

            Kilian E.C. Smith

5. The In Vitro Micronucleus Assay and FISH Analysis

            Lucia Migliore, Sebastiano Di Bucchianico, and Chiara Uboldi

6. The In Vivo Rodent Micronucleus Test

            Edson Luis Maistro

7. Chromosomal Aberration Test Utilities In Vitro and In Vivo

            Ana Paula A. Guimarães, Adriana C. Guimarães, Diego Á. Alcântara, Luiz Raimundo Cunha, Patrícia L. Lima, Marne C. Vasconcellos, Raquel C. Montenegro, Bruno M. Soares, Marucia M. Amorim, and Rommel R. Burbano

8. Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues

            Dayton M. Petibone, James D. Tucker, and Suzanne M. Morris

9. T-Cell Receptor Mutation Assay for Monitoring Human Genotoxic Exposure

            Seishi Kyoizumi

10. The Human RBC PIG-A Gene Mutation Assay

            Vasily N. Dobrovolsky and Robert H. Heflich

11. The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies

            Zoulikha M. Zaïr and George E. Johnson

12. The Comet Assay: High Throughput Use of FPG

            Amaya Azqueta and Andrew R. Collins

13. The Comet Assay In Vivo in Humans

            Carla Costa and João Paulo Teixeira

14. Analysis of Nanoparticle-Induced DNA Damage by the Comet Assay

            Julia Catalán, Satu Suhonen, Anna Huk, and Maria Dusinska

15. The Comet Assay in Drosophila: Neuroblast and Haemocyte Cells

            L. María Sierra, Erico R. Carmona, Leticia Aguado, and Ricard Marcos

16. The SMART Assays of Drosophila: Wings and Eyes as Target Tissues

            Ricard Marcos, L. María Sierra, and Isabel Gaivão

17. Testing the Genotoxic Potential of Nanomaterials Using Drosophila

            Mohamed A. Abdalaziz, Balasubramanyam Annangi, and Ricard Marcos

18. Transgenic Rodent Gene Mutation Assay in Somatic Tissues

            John D. Gingerich, Lynda Soper, Christine L. Lemieux, Francesco Marchetti, and George R. Douglas

19. The Mouse Lymphoma Assay

            Tao Chen, Xiaoqing Guo, and Martha M. Moore

20. Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens

            Kiyoshi Sasaki, Anna Huk, Naouale El Yamani, Noriho Tanaka, and Maria Dusinska

Part II: DNA Repair Assays

21. Methods for Measuring DNA Repair: Introduction and Cellular Repair

            Andrew R. Collins and Amaya Azqueta

22. A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay

            Jana Slyskova, Sabine A.S. Langie, Isabel Gaivão, Andrew R. Collins, and Amaya Azqueta

23. Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster

            Isabel Gaivão, Rubén Rodríguez, and L. María Sierra

24. Use of RNA Interference to Study DNA Repair

            Elise Fouquerel, Jianfeng Li, Andrea Braganza, Zhongxun Yu, Ashley R. Brown, Xiao-hong Wang, Sandy Schamus, David Svilar, Qingming Fang, and Robert W. Sobol

Part III: Others

25. The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials

            Henriqueta Louro, Miguel Pinto, Nádia Vital, Ana M. Tavares, Pedro M. Costa, and Maria João Silva