Andrew Hill, Associate Professor/Principle Research Fellow, Department of Biochemistry and Molecular Biology, The University of Melbourne Bio21 Institute, 
Email: a.hill@unimelb.edu.au

Preface...
Table of Contents...
Contributing Authors...

1. Methods to Analyze EVs
Bernd Giebel and Clemens Helmbrecht
2. Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles
Sybren L. N. Maas, Marike L.D. Broekman, and Jeroen de Vrij
3. Immuno-Characterization of Exosomes using Nanoparticle Tracking Analysis
Kym McNicholas and Michael Z. Michael
4. Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy
Romain Linares, Sisareuth Tan, Céline Gounou, and Alain R. Brisson
5. Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR
Shayne A. Bellingham, Mitch Shambrook, and Andrew F. Hill
6. Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM(TM) System
Lesley Cheng and Andrew F. Hill
7. A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration
Rong Xu, Richard J. Simpson, and David W. Greening
8. Multiplexed Phenotyping of Extracellular Vesicles using Protein Microarray (EV Array)
Rikke Bæk and Malene Møller Jørgensen
9. Purification and Analysis of Exosomes Released by Mature Cortical Neurons following Synaptic Activation
Karine Laulagnier, Charlotte Javalet, Fiona Hemming, and Rémy Sadoul
10. A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space
R. Perez-Gonzalez, S.A. Gauthier, A. Kumar, M. Saito, M. Saito, and E. Levy
11. Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission
Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann, Laurent Schaeffer, Graça Raposo and Didier Vilette
12. Isolation of Platelet-Derived Extracellular Vesicles
Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland and Pia Siljander
13. Bioinformatics Tools for Extracellular Vesicles Research
Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, and Suresh Mathivanan
14. Preparation and Isolation of siRNA-Loaded Extracellular Vesicles
Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir EL Andaloussi, and Matthew J.A. Wood
15. Interaction of Extracellular Vesicles with Endothelial Cells under Physiological Flow Conditions
S.M. van Dommelen, M. Fish, A.D. Barendrecht, R.M. Schiffelers, O. Eniola-Adefeso, P. Vader