This detailed volume provides a single, valuable reference source for
methods that definitively identify and accurately quantify apoptosis. The book
begins with common methods utilized to detect and quantitate apoptosis, as well
as apoptosis signaling pathways in toxicological and other related research. It
continues with multi-parametric and phased apoptosis assays for detecting early
and late apoptosis or distinguishing apoptosis from necrosis and autophagy.
Subsequent chapters focus on recent advances in real time and high-throughput
assays that detect and quantitate apoptosis and apoptosis signaling pathways.
Final chapters focus on recent developments in preclinical anticancer
therapeutics targeting apoptosis. Written for the Methods in Pharmacology
and Toxicology series, chapters feature step-by-step descriptions of the
methodologies, as well as expert tips and implementation advice.
Vital and authoritative, Apoptosis Methods in
Toxicology serves novice scientists as well as experts,
utilizing a range of instruments from common laboratory equipment to high-end
expensive and automated machinery capable of performing real time apoptotic
measurements.

1. An Overview of
Apoptosis Methods in Toxicological Research: Recent Updates
            Perpetua M.
Muganda
 
2. Detection of Apoptosis: From Bench Side
to Clinical Practice
            William P.
Bozza, Julianne D. Twomey, Su-Ryun Kim, and Baolin Zhang
 
3. Determining the
Extent of Toxicant-Induced Apoptosis Using Concurrent Phased Apoptosis Assays
            Akamu Jude
Ewunkem and Perpetua M. Muganda
 
4. Measurement of Apoptosis
by Multiparametric Flow Cytometry
            William
Telford
 
5. Detecting
Apoptosis, Autophagy, and Necrosis
            Jack
Coleman, Rui Liu, Kathy Wang, and Arun Kumar
 
6. A Low-Cost Method
for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in
Suspension Cells
            Akamu J.
Ewunkem, Carl D. Parson II, Perpetua M. Muganda, and Robert H. Newman
 
7. FRET-Based
Measurement of Apoptotic Caspase Activities by High-Throughput Screening Flow
Cytometry
            Christian T. Hellwig, Agnieszka H.
Ludwig-Galezowska, and Markus Rehm
 
8. Automated
Ratio Imaging Using Nuclear-Targeted Fret Probe-Expressing Cells for Apoptosis
Detection
            Krupa Ann
Mathew, Deepa Indira, Jeena Joseph, Prakash Rajappan Pillai, Indu Ramachandran,
Shankara Narayanan Varadarajan, and Santhoshkumar Thankayyan Retnabai
 
9. Antibody-Based Proteomic Analysis of
Apoptosis Signaling
            Matthew P. Stokes, Hongbo Gu, and
Jeffrey C. Silva
 
<10. Novel Electrochemical Biosensor for
Apoptosis Evaluation
            Peng Miao and Jian Yin
 
11. Microplate-Based Whole Zebrafish Caspase 3/7 Assay
for Screening Small Molecule Compounds         
            Wen Lin Seng, Dawei Zhang, and
Patricia McGrath
 
12. Targeting Cancer Cell Death with Small-Molecule Agents
for Potential Therapeutics
            Lan Zhang, Yaxin Zheng, Mao Tian,
Shou-yue Zhang, Bo Liu, and Jinhui Wang
 
13. Liposomes in Apoptosis Induction and Cancer Therapy
            Magisetty Obulesu and
Magisetty Jhansilakshmi