This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes. Lipidomics guides readers through chapters on direct-flow and chromatographic methods (SFC, UHPLC, HPTLC, ion-mobility); derivatization methods for lipids (amines, fatty aldehydes and ketones); TOF-SIMS imaging of lipids; and characterization of lipid transfer proteins. Additional chapters also provide an authoritative overview of lipidomics strategies and a detailed review of high-resolution mass spectrometric methods are included in this volume. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Lipidomics aims to ensure successful results in the further study of this vital field.

1. Introduction and Overview of Lipidomic Strategies
William J. Griffiths and Yuqin Wang
 
2. Non-targeted Lipidomics Utilizing Constant Infusion High Resolution ESI Mass Spectrometry
Paul L. Wood
 
3. Ultrahigh-Resolution Lipid Analysis with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Mark R. Emmett and Cheryl F. Lichti
 
4. MALDI-Imaging Mass Spectrometry of Brain Lipids
Norelle C. Wildburger
 
5. Ion-Mobility Mass Spectrometry for Lipidomics Applications
Giuseppe Paglia, Bindesh Shrestha, and Giuseppe Astarita
 
6. In vitro Assay to Extract Specific Lipid Types from Phospholipid Membranes using Lipid-Transfer Proteins: A Lesson from the Ceramide Transport Protein CERT
Kentaro Hanada and Toshihiko Sugiki
 
7. Quantification of Endogenous Endocannabinoids by LC-MS/MS
Mesut Bilgin and Andrej Shevchenko
 
8. Lipid Profiling by Supercritical Fluid Chromatography/Mass Spectrometry
Takayuki Yamada and Takeshi Bamba
 
9. Mass Spectrometric Analysis of Lipid Hydroperoxides
Tânia Melo, Elisabete Maciel, Ana Reis, Pedro Domingues, and M. Rosário M. Domingues
 
10. Mass Spectrometric Determination of Fatty Aldehydes Exemplified by Monitoring the Oxidative Degradation of (2E)-hexadecenal in HepG2 Cell Lysates
Corinna Neuber, Fabian Schumacher, Erich Gulbins, and Burkhard Kleuser
  11. CE Analysis of Phospholipid Headgroups Václav Mat¿j Bierhanzl, Martina Riesová, Gabriela Seydlová and Radomír ¿abala
 
12. HPTLC-MALDI TOF MS Imaging Analysis of Phospholipids
Tatiana Kondakova, Nadine Merlet Machour, and Cécile Duclairoir Poc
 
13. Global UHPLC/HRMS Lipidomics Workflow for the Analysis of Lymphocyte Suspension Cultures
Candice Z. Ulmer, Richard A. Yost, and Timothy J. Garrett
 
14. Ambient Lipidomic Analysis of Brain Tissue using Desorption Electrospray Ionization (DESI) Mass Spectrometry
Valentina Pirro, Alan K. Jarmusch, Christina R. Ferreira, and R. Graham Cooks
15. Lipidomics Analyses of Oxygenated Metabolites of Polyunsaturated Fatty Acids Alexandra C Kendall and Anna Nicolaou
 
16. Derivatization of Fatty Aldehydes and Ketones: Girard¿s Reagent T (GRT)
Paul L. Wood
  17. Derivatization of Lipid Amines: Fluorenylmethyloxycarbonyl (FMOC) Paul L. Wood
  18. High-Resolution Mass Spectrometry of Glycerophospholipid Oxidation Products Paul L. Wood