A cutting-edge collection of readily reproducible molecular techniques to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for providing for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question.

Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins.- Molecular Diagnosis of Lymphoma.- Demonstration of a Germinal Center Immunophenotype in Lymphomas by Immunocytochemistry and Flow Cytometry.- Karyotyping Lymph Node Biopsies in Non-Hodgkin's Lymphoma.- Identification of Lymphoma-Associated Antigens Using SEREX.- Determining Mutational Status of Immunoglobulin V Genes in Chronic Lymphocytic Leukemia.- Idiotype Gene Rescue in Follicular Lymphoma.- Immunoglobulin Gene Mutation Patterns and Heterogeneity of Marginal Zone Lymphoma.- T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma.- Cloning of Immunoglobulin Chromosomal Translocations by Long-Distance Inverse Polymerase Chain Reaction.- Sequential Fluorescence In Situ Hybridization Analysis for Trisomy 12 in B-Cell Chronic Lymphocytic Leukemia.- Splenic Marginal Zone Lymphoma.- BCL-6.- Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL.- Identification of Anaplastic Lymphoma Kinase Variant Translocations Using 5?RACE.- Follicular Lymphoma.