Part I. Molecular Characterization of Opioid Receptors and Signaling Pathways

Molecular Cloning of Opioid Receptors by cDNA Library Screening
Ying-Xian Pan

Expression of Opioid Receptors in Mammalian Cell Lines
Ying-Xian Pan

Assessing Opioid Regulation of Adenylyl Cyclase Activity in Intact Cells
Deepak R. Thakker, Hatice Z. Ozsoy, and Kelly M. Standifer

Analysis of Opioid-Induced Kinase Activation, Lan Ma

Study of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-Orthrophosphate
Jia Bei Wang

Opioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant Mice
Kazutaka Ikeda, Mitsunobu Yoshii, Ichiro Sora, and Toru Kobayashi

Identification of Alternatively Spliced Variants from Opioid Receptor Genes
Ying-Xian Pan

Part II. Mapping and Detection of Endogenous Opioids

Immunohistochemical Localization of m-, d- and k-Opioid Receptors Within the Antinociceptive Brainstem Circuits
Alexander E. Kalyuzhny

In Situ Hybridization in Neural Tissues
Howard B. Gutstein

Quantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping Genes
Seth C. Silbert

Gene Arrays and Proteomics: A Primer
Lionel Moulédous and Howard B. Gutstein

Part III. Model Systems for Studies on Opioid Functions

Opioid Receptor Oligomerization: Detection and Functional Characterization of Interacting Receptors
Ivone Gomes, Julija Filipovska, and Lakshmi A. Devi

Recombinant Opioid Receptors: Structure-Function Relationship
Julija Filipovska, Ivone Gomes, Wei Xu, Chongguang Chen, Lee-Yuan Liu-Chen, and Lakshmi A. Devi

Receptor Knock-Out and Gene Targeting: Generation of Knock-Out Mice
Ichiro Sora, Kazutaka Ikeda, and Yuji Mishina

Pharmacological Interventions at theSpinal Cord: Intrathecal Injections
Daphné A. Robinson and Min Zhuo

Opioid Tolerance in Adult and Neonatal Rats
Zhizhong Z. Pan

Animal Models of Neuropathic Pain
William J. Martin, Laike St. A. Stewart, and Jason W. Tarpley

Place Conditioning to Study Drug Reward and Aversion
William A. Carlezon, Jr.

Opiate Self-Administration
Zheng-Xiong Xi and Elliot A. Stein

Part IV. Clinical Applications of Opioids

Acute, Chronic, and Cancer Pain: Clinical Management
Allen W. Burton

Clinical Treatment of Opioid Addiction and Dependence
Walter Ling, Richard A. Rawson and Margaret Compton

Index