Part I. Tissue Techniques

Morphology and Molecular Properties of Cellular Components of Normal Cerebral Vessels
Sukriti Nag

Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry
Sukriti Nag

Freeze-Fracture Studies of Cerebral Endothelial Tight Junctions
Hartwig Wolburg, Stefan Liebner, and Andrea Lippoldt

Studies of Cerebral Endothelium by Scanning and High-Voltage Electron Microscopy
Albert S. Lossinsky and Richard R. Shivers

Detection of Endothelial/Lymphocyte Interaction in Spinal Cord Microvasculature by Intravital Videomicroscopy
Britta Engelhardt, Peter Vajkoczy, and Melanie Laschinger

Part II. Blood-Brain Barrier Permeability Techniques

Pathophysiology of Blood-Brain Barrier Breakdown
Sukriti Nag

Pial Vessel Permeability to Tracers Using Cranial Windows
William G. Mayhan

Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
Sukriti Nag

Ultracytochemical Studies of the Compromised Blood-Brain Barrier
Sukriti Nag

Immunogold Detection of Microvascular Proteins in the Compromised Blood-Brain Barrier
Eain M. Cornford, Shigeyo Hyman, and Marcia E. Cornford

Measurement of Blood-Brain and Blood-Tumor Barrier Permeabilities with [14C]-Labeled Tracers
Kamlesh Asotra, Nagendra Ningaraj, and Keith L. Black

Part III. Blood-Brain Barrier Transport Techniques

A Review of Blood-Brain Barrier Transport Techniques
Quentin R. Smith

In Situ Brain Perfusion Technique
Quentin R. Smith and David D. Allen

Brain Efflux Index Method: Characterization of Efflux Transport Across the Blood-Brain Barrier
Hiroyuki Kusuhara, Tetsuya Terasaki, and Yuichi Sugiyama

Transport Studies Using Membrane Vesicles
Darryl R. Peterson and Richard A. Hawkins

Drug Transport Studies Using Quantitative Microdialysis
Haiqing Dai and William F. Elmquist

Single-Pass Dual-Label Indicator Method: Blood-to-Brain Transport of Glucose and Short-Chain Monocarboxylic Acids
Michelle A. Puchowicz, Kui Xu, and Joseph C. LaManna

Protein Transport in Cerebral Endothelium: In Vitro Transcytosis of Transferrin
Laurence Fenart and Roméo Cecchelli

Transport Across the Choroid Plexuses In Vivo and In Vitro
Nathalie Strazielle and Jane Elizabeth Preston

Part IV. In Vitro Techniques

An Overview of In Vitro Techniques for Blood-Brain Barrier Studies
Andreas Reichel, David J. Begley, and N. Joan Abbott

Isolation and Characterization of Human Brain Endothelial Cells
Katerina Dorovini-Zis, Rukmini Premeya, and Hanh Huynh

Human Brain Microvessel Endothelial Cell and Leukocyte Interactions
Jacqueline Shukaliak-Quandt, Donald Wong, and Katerina Dorovini-Zis

Development and Characterization of Immortalized Cerebral Endothelial Cell Lines
Pierre-Olivier Couraud, John Greenwood, Françoise Roux, and Pete Adamson

Isolation and Characterization of Retinal Endothelial Cells
David A. Antonetti and Ellen B. Wolpert

Isolation and Characterization of Cerebral Microvascular Pericytes
Paula Dore-Duffy

Part V. Molecular Techniques

Molecular Biology of the Blood-Brain Barrier
William M. Pardridge

Blood-Brain Barrier Genomics
Ruben J. Boado

Dynamic In Vitro Model of the Blood-Brain Barrier: Gene Profiling Using cDNA Microarray Analysis
Matteo Marroni, Kelly M. Kight, Mohammed Hossain, Luca Cucullo, Shailesh Y. Desai, and Damir Janigro

Phage Display Technology for Identifying Specific Antigens on Brain Endothelial Cells
Jamshid Tanha, Arumugam Muruganandam, and Danica Stanimirovic

mRNA Detection in Cerebral Vessels by Nonradioactive In Situ