Part I. Preparation of Cell and Tissue Samples

Breast Tissue Banking: Collection, Handling, Storage, and Release of Tissue for Breast Cancer Research
Linda Snell and Peter H. Watson

Frozen Biopsy Collection and Storage: Frozen Biopsy Samples
Russell Leek

Serial Biopsies/Fine-Needle Aspirates and Their Assessment
Irene Boeddinghaus and Stephen R. D. Johnson

Breast Tissue Microarrays
Robert J. Springall and Cheryl E. Gillett

Preparation of Tumor Homogenates for Subsequent Preparation of Cytosols, Membrane Fractions, RNA, and DNA
Naomi Robertson and Russell Leek

Isolation of RNA From Tumor Samples: Single-Step Guanidinium Acid-Phenol Method
Naomi Robertson and Russell Leek

Isolation of DNA From Tumor Samples
Naomi Robertson and Russell Leek

Laser-Assisted Microdissection and Isolation of DNA and RNA
Ulrich Lehmann and Hans Kreipe

Part II. Markers of Clinical Outcome and Prognosis

Traditional and Established Indicators of Prognosis and Treatment Success
Derek E. Roskell and Ian D. Buley

Sentinel Lymph Node Biopsy in Breast Cancer
Celia Chao and Kelly M. McMasters

Pathological Evaluation of Axillary Sentinel Lymph Nodes in Breast Cancer
Michael Z. Gilcrease and Aysegul Sahin

Measurement of Estrogen Receptor Status by Immunocytochemistry in Paraffin Wax Sections
Bharat Jasani, Anthony Douglas-Jones, Anthony Rhodes, Susan Wozniak, Peter J. Barrett-Lee, Julia Gee, and Robert Nicholson

Markers of Apoptosis
Fazlul H. Sarkar and Yiwei Li

Quantitative Angiogenesis in Breast Cancer
Stephen B. Fox

Part III. Analysis of Tumor-Derived Proteins and Antigens

Immunohistochemistry
Cheryl E. Gillett

Detection of Aberrant Glycosylation in Breast Cancer Using Lectin Histochemistry
Tracey M. Carter and Susan A. Brooks

SDS-PAGE and Western Blotting to Detect Proteins and Glycoproteins of Interest in Breast Cancer Research
Chloe Osborne and Susan A. Brooks

Breast Cancer Proteomics Using Two-Dimensional Electrophoresis: Studying the Breast Cancer Proteome
Miriam V. Dwek and Sarah L. Rawlings

Procedures for the Quantitative Protein Determination of Urokinase and Its Inhibitor, PAI-1, in Human Breast Cancer Tissue Extracts by ELISA
Manfred Schmitt, Alexandra S. Sturmheit, Anita Welk, Christel Schnelldorfer, and Nadia Harbeck

Part IV. Analysis of Genes and Gene Expression in Tumor Specimens

Fluorescence In Situ Hybridization and Comparative Genomic Hybridization
Patricia Gorman and Rebecca Roylance

Fluorescence In Situ Hybridization Assessment of c-myc Gene Amplification in Breast Tumor Tissues
Jan K. Blancato, Mary Steele Williams, and Robert B. Dickson

Detection of HER2 Gene Amplification by Fluorescence In Situ Hybridization in Breast Cancer
John M. S. Bartlett and Amanda Forsyth

In Situ Hybridization Combined With Immunohistochemistry to Localize Gene Expression
Rosemary Jeffery, Toby Hunt, and Richard Poulsom

Quantitation of RNA by Ribonuclease Protection Assay
John W. Moore

Identification of Steroid Hormone-Regulated Genes in Breast Cancer
Bruce R. Westley and Felicity E. B. May

Sequencing of the Tumor Suppressor Gene TP 53
Barbro Linderholm, Torbjörn Norberg, and Jonas Bergh

Expression Profiling Using cDNA Microarrays
Chris Jones, Peter Simpson, Alan Mackay, and Sunil R. Lakhani

Gene Expression Analysis Using Filter cDNA Microarrays
Peter Simpson, Chris Jones, Alan Mackay, and Sunil R. Lakhani

Part V. Studying Cancer Cell Behavior In Vitro and In Vivo

Methods to Analyze the Effects of the Urokinase System on Cancer Cell Adhesion, Proliferation, Migration,