1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester
2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Alastair S. Garfield
3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells
Janet Smith and Deborah Merrick
4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference
Jamie A. Davies
5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation
Pierre-Marie del Moral and David Warburton
6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh
7. Isolation and Culture of Embryonic Pancreas and Liver
Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh
8. Isolation and Culture of Mouse Satellite Cells
Antonio Musarò and Laura Barberi
9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures
Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton
10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
Reto Caldelari and Eliane J. Müller
11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells
Matthew J. Smalley
12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes
Magalie A. Ravier and Guy A. Rutter
13. Isolation and Culture of Adult Mouse Hepatocytes
Wan-ChunLi, Kate L. Ralphs, and David Tosh
14. Isolation and Culture of Mouse Intestinal Cells
F. Charles Campbell
15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse
Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward
16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry
Carla Sciarretta and Liliana Minichiello
17. Cell Culture of Primary Cerebellar Granule Cells
Dana Krämer and Liliana Minichiello
18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Henrik Ahlenius and Zaal Kokaia