Bültmann & Gerriets
Glycomics
Methods and Protocols
von Nicolle H. Packer, Niclas G. Karlsson
Verlag: Humana Press
Reihe: Methods in Molecular Biology Nr. 534
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ISBN: 978-1-59745-022-5
Auflage: 2009
Erschienen am 11.03.2009
Sprache: Englisch
Umfang: 390 Seiten

Preis: 96,29 €

Inhaltsverzeichnis

Section I: Glycan Analysis
Subsection A: Released Glycans
1. Analysis of N- and O-Linked Glycans from Glycoproteins Using Maldi-TOF Mass Spectrometry
Willy Morelle, Valegh Faid, Frédéric Chirat, and Jean-Claude Michalski
2. Infrared Multiphoton Dissociation (IRMPD) Mass Spectrometry for Structural Elucidation of Oligosaccharides
Bensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla
3. Mass Spectrometry of N-linked Glycans
Parastoo Azadi and Christian Heiss
4. Solid-phase Permethylation for Glycomic Analysis
Yehia Mechref, Pilsoo Kang, and Milos V. Novotny
5. Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization
Erika Lattová and Hélène Perreault
6. 2-Dimensional HPLC Separation with Reverse Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools after Labeling with 2-Aminobenzamide
Manfred Wuhrer, Carolien A. M. Koeleman, and André M. Deelder
7. Capillary Lectin-Affinity Electrophoresis for Glycan Analysis
Kazuaki Kakehi and Mitsuhiro Kinoshita
8. Analysis of Methylated O-Glycan Alditols by CAD-ESI Mass Spectrometry
Franz-Georg Hanisch and Stefan Müller
9. High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling
Hasse Karlsson, Jessica M. Holmén Larsson, Kristina A. Thomsson, Iris Härd, Malin Bäckström, and Gunnar C. Hansson
10. Collision Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans
Bensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla
11. The Structural Elucidation of Glycosaminoglycans
Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan
12. Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescenceand Mass Tags for HPLC and MS Separations
Mark Skidmore, Abdel Atrih, Ed Yates, and Jeremy E. Turnbull
13. Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis
Ruby P. Estrella, John M. Whitelock, Rebecca H. Roubin, Nicolle H. Packer, and Niclas G. Karlsson

Subsection B: Glycopeptides
14. Enrichment Strategies for Glycopeptides
Shigeyasu Ito, Ko Hayama, and Jun Hirabayashi
15. Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting
Nicole Wilson, Raina Simpson, and Catherine Cooper-Liddell
16. Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF
Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim, and Tillman Gerngross
17. N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS
Urs Lewandrowski and Albert Sickmann
18. LC/MSn For Glycoprotein Analysis: N-Linked Glycosylation Analysis And Peptide Sequencing Of Glycopeptides
Nana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi

Subsection C: O-GlcNAcomics
19. Detecting the 'O-GlcNAc-ome'; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins
Natasha E. Zachara

Subsection D: Glycobioinformatic Tools
20. Preparation of a Glycan Library Using a Variety of Glycosyltrasferases
Hiromi Ito, Akihiko Kameyama, Takashi Sato, and Hisashi Narimatsu
21. Data Mining the PDB for Glyco-Related Data
Thomas Lütteke and Claus-W. von der Lieth

Section II: Carbohydrate-Protein


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