Human Embryonic Stem Cells: Isolation, Maintenance, and Differentiation
Kursad Turksen and Tammy-Claire Troy

Blastocyst Culture for Deriving Human Embryonic Stem Cells
Ariff Bongso

Propagation of Human Embryonic Stem Cells on Human Feeder Cells
Mark Richards and Ariff Bongso

Derivation and Maintenance of Human Embryonic Stem Cells
Michal Amit and Joseph Itskovitz-Eldor

Isolation and Characterization of Human Embryonic Stem Cells
Maisam Mitalipova and Gail Palmarini

Routine Culture and Differentiation of Human Embryonic Stem Cells
Jim McWhir, Davina Wojtacha, and Alison Thomson

Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells
Gautam Dravid, Holly Hammond, and Linzhao Cheng

Maintenance of Human Embryonic Stem Cells in Animal Serum- and Feeder Layer-Free Culture Conditions
Michal Amit and Joseph Itskovitz-Eldor

Manipulation of Self-Renewal in Human Embryonic Stem Cells Through a Novel Pharmacological GSK-3 Inhibitor
Noboru Sato and Ali H. Brivanlou

Derivation of Human Feeders for Prolonged Support of Human Embryonic Stem Cells
Chui-Yee Fong and Ariff Bongso

Transplantation of Human Embryonic Stem Cells to the Chick Embryo
Ronald S. Goldstein

Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic Stem Cells In Vitro
Melissa Carpenter, Mahendra S. Rao, William J. Freed, and Xianmin Zeng

In Vitro Differentiation of Neural Precursors From Human Embryonic Stem Cells
Xue-Jun Li and Su-Chun Zhang

Derivation and Characterization of Hematopoietic Cells From Human Embryonic Stem Cells
Lisheng Wang, Chantal Cerdan, Pablo Menendez, and Mickie Bhatia

Retroviral Transduction of Hematopoietic Progenitors Derived From Human Embryonic Stem Cells
Pablo Menendez, Lisheng Wang, Chantal Cerdan, andMickie Bhatia

Genetic Manipulation of Human Embryonic Stem Cells by Transfection
Rachel Eiges

Designing, Testing, and Validating a Microarray for Stem Cell Characterization
Yongquan Luo, Bhaskar Bhattacharya, Amy X. Yang, Raj K. Puri, and Mahendra S. Rao

Microarray Approach to Identify the Signaling Network Responsible for Self-Renewal of Human Embryonic Stem Cells
Noboru Sato and Ali H. Brivanlou

Massively Parallel Signature Sequencing
Daixing Zhou, Mahendra S. Rao, Roger Walker, Irina Khrebtukova, Christian D. Haudenschild, Takumi Miura, Shannon Decola, Eric Vermaas, Keith Moon, and Thomas J. Vasicek

Confrontation Cultures of Embryonic Stem Cells With Multicellular Tumor Spheroids to Study Tumor-Induced Angiogenesis
Maria Wartenberg, Andreas Finkensieper, Jürgen Hescheler, and Heinrich Sauer

A Novel Experimental Platform for Investigating Cancer Growth and Anti-Cancer Therapy in a Human Tissue Microenvironment Derived From Human Embryonic Stem Cells, Maty Tzukerman and Karl L. Skorecki

The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
Justin C. St. John, Alexandra Amaral, Emma Bowles, João Facucho Oliveira, Rhiannon Lloyd, Mariana Freitas, Heather L. Gray, Christopher S. Navara, Gisela Oliveira, Gerald P. Schatten, Emma Spikings, and João Ramalho-Santos

Index