New Antibiotic Targets Book Chapter Titles 1. Biocomputational Strategies for Microbial Drug Target Identification 2. Methods to Assay Inhibitors of DNA Gyrase and Topoisomerase IV Activities 3. A Method to Assay Inhibitors of DNA Polymerase III Activity 4. Methods to Identify and Characterize Inhibitors of Bacterial RNA Polymerase 5. Methods to Assay Inhibitors of tRNA Synthetase Activity 6. Three Methods to Assay Inhibitors of Ribosomal Subunit Assembly 7. Inhibition of Chaperone-dependent Bacterial Ribosome Biogenesis 8. Assays for the Identification of Inhibitors Targeting Specific Translational Steps 9. SPARK - A New Peptidyl Transferase Activity Assay 10. High-throughput Screening of Peptide Deformylase Inhibitors 11. A Method to Assay Penicillin Binding Proteins 12. A Method to Assay Inhibitors of Lipopolysaccharide Synthesis 13. Methods for Assessing the Structure and Function of Cationic Antimicrobial Peptides 14. Flow Cytometry of Bacterial Membrane Potential and Permeability 15. Bacterial Efflux Pump Inhibitors 16. Mycobacterium tuberculosis Beta-ketoacyl Acyl Carrier Protein Synthase III (mtFabH) Assay - Principle and Method 17. Screening for Compounds that Affect the Interaction between Bacterial Two- Component Signal Transduction Response Regulator Protein and Cognate Promoter DNA 18. The Activity of rRNA Resistance Methyltransferases Assessed by MALDI Mass Spectrometry 19. Assays for Beta-lactamase Activity and Inhibition 20. Studies of Enzymes that Cause Resistance to Aminoglycoside Antibiotics