Part I: Biology of the Barriers
1. Morphology and Properties of Brain Endothelial Cells
Sukriti Nag
2. Morphology and Properties of Pericytes
Paula Dore-Duffy and Kristen Cleary
3. Morphology and Properties of Astrocytes
Sukriti Nag
4. The Blood-Cerebrospinal Fluid Barrier: Structure and Functional Significance
Conrad E. Johanson, Edward G. Stopa, and Paul N. McMillan
5. The Blood-Retinal Barrier: Structure and Functional Significance
E. Aaron Runkle and David A. Antonetti
6. The Blood-Nerve Barrier: Structure and Functional Significance
Ananda Weerasuriya and Andrew P. Mizisin
Part II: Imaging the Barriers
7. Detection of Multiple Proteins in Intracerebral Vessels by Confocal Microscopy
Janet L. Manias, Anish Kapadia, and Sukriti Nag
8. Multiparametric Magnetic Resonance Imaging and Repeated Measurements of Blood-Brain Barrier Permeability to Contrast Agents
Tavarekere N. Nagaraja, Robert A. Knight, James R. Ewing, Kishor Karki, Vijaya Nagesh, and Joseph D. Fenstermacher
9. Detection of Brain Pathology by Magnetic Resonance Imaging of Iron Oxide Microparticles
Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis, and Robin P. Choudhury
10. Measuring the Integrity of the Human Blood-Brain Barrier Using Magnetic Resonance Imaging
Andrea Kassner and Rebecca Thornhill
11. Assessing Blood-Cerebrospinal Fluid Barrier Permeability in the Rat Embryo
Norman R. Saunders, C. Joakim Ek, Mark D. Habgood, Pia Johansson, Shane Liddelow, and Katarzyna M. Dziegielewska
12. Detection of Blood-Nerve Barrier Permeability by Magnetic Resonance Imaging
Carsten Wessig
Part III: Molecular Techniques to Study the Blood-Brain Barrier
13. Isolation of Human Brain Endothelial Cells and Characterization of Lipid Raft-Associated Proteins by Mass Spectroscopy
Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers, and Alexandre Prat
14. Analysis of Mouse Brain Microvascular Endothelium Using Laser Capture Microdissection Coupled with Proteomics
Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan Lu, Shiaw-Lin Wu, William S. Hancock, and Joel S. Pachter
15. Molecular and Functional Characterization of P-Glycoprotein In vitro
Gary N.Y. Chan and Reina Bendayan
16. Methods to Study Glycoproteins at the Blood-Brain Barrier Using Mass Spectrometry
Arsalan S. Haqqani, Jennifer J. Hill, James Mullen, and Danica B. Stanimirovic
Part IV: Models to Study the Barriers
17. Novel Models for Studying the Blood-Brain and Blood-Eye Barriers in Drosophila
Robert L. Pinsonneault, Nasima Mayer, Fahima Mayer, Nebiyu Tegegn, and Roland J. Bainton
18. Zebrafish Model of the Blood-Brain Barrier: Morphological and Permeability Studies
Brian P. Eliceiri, Ana Maria Gonzalez, and Andrew Baird
19. Methods to Assess Pericyte-Endothelial Cell Interactions in a Co-Culture Model
Gokulan Thanabalasundaram, Jehad El-Gindi, Mira Lischper, and Hans-Joachim Galla
20. Isolation and Properties of an In vitro Human Outer Blood-Retinal Barrier Model
Robin D. Hamilton and Lopa Leach
21. Isolation and Properties of Endothelial Cells Forming the Blood-Nerve Barrier
Yasuteru Sano and Takashi Kanda
Part V: Delivery of Therapeutic Agents Across the Barriers
22. Treatment of Focal Brain Ischemia with Viral Vector-Mediated Gene Transfer
Hua Su and Guo-Yuan Yang
23. Blood-Brain Barrier Disruption in the Treatment of Brain Tumors
Marie Blanchette and David Fortin
24. Integrated Platform for Brain Imaging and Drug Delivery Across the Blood-Brain Barrier
Umar Iqbal, Abedelnasser Abulrob, and Danica B. Stanimirovic
25. Targeting the Choroid Plexus-CSF-Brain Nexus Using Peptides Identified by Phage Display
Andrew Baird, Brian P. Eliceiri, Conrad E. Johanson, Edward G. Stopa, Wendy Leadbeater, and Ana Maria Gonzalez