An outstanding collection of state-of-the-art experimental methods for herpes virology. These procedures range from the more biological in vivo maneuvers to the purely molecular, and are described in detail by selected experts to ensure reproducibility. Most of the protocols rely on the herpes simplex virus as prototype, so that it then becomes relatively easy to
extrapolate and make the necessary modifications required for application to other herpesviruses, especially members of the alpha group, such as PRV and EHV.

HSV Growth, Preparation, and Assay, June Harland and S. Moira Brown. HSV
Entry and Spread, Christine A. MacLean. Preparation of HSV-DNA and
Production of Infectious Virus, Alasdair R. MacLean. HSV Mutagenesis, Robert
S. Coffin. Saturating Mutagenesis and Characterization of a Herpesvirus
Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions,f
Niels de Wind, Maddy van Zijl, and Anton Berns. Construction and Use of Cell
Lines Expressing HSV Genes, Claire Entwisle. Analysis of HSV Polypeptides,
Lars Haarr and Nina Langeland. Protein Purification, Joseph Conner.
Expression and Purification of Secreted Forms of HSV Glycoproteins from
Baculovirus-Infected Insect Cells, Sharon H. Willis, Charline Peng, Manuel
Ponce de Leon, Anthony V. Nicola, Ann H. Rux, Gary H. Cohen, and Roselyn J.
Eisenberg. Crystallization of Macromolecules for Three-Dimensional Structure
Determination, Ben Luisi, Marie Anderson, and Graham Hope. Direct Immunogold
Labeling of Herpesvirus Suspensions, Linda M. Stannard. Expression of HSV
Proteins in Bacteria, Elizabeth A. McKie. In Vitro Systems to Analyze HSV
Transcript Processing, Anne Phelan and J. Barklie Clements. Analysis of
HSV-1 Transcripts by RNA-PCR, Jordan G. Spivack. Transient Assays for HSV
Origin and Replication Protein Function, Nigel D. Stow. HSV Amplicons in
Gene Therapy, Niza Frenkel and Ronit Sarid. Analyses of HSV Proteins for
Posttranslational Modifications and Enzyme Functions, John A. Blaho and
Bernard Roizman. HSV-Cellular Protein Interactions, David S. Latchman.
Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse,
Terry J. Hill and Carolyn Shimeld. Pathogenesis and Molecular Biology of HSV
Latency and Ocular Reactivation in the Rabbit, James M. Hill, Renjie Wen,
and William P. Halford. HSV Latency In Vitro: In Situ Hybridization Methods,
Christine L. Wilcox and R.L. Smith. Assessing Cell-Mediated Immune
Responses to HSV in Murine Systems, Theresa A. Banks, Mangala J. Hariharan,
and Barry T. Rouse. Assays for HSV Gene Expression During Establishment and
Maintenance of Latent Infection, Peter Speck and Stacey Efstathiou. Analysis
of HSV-DNA and RNA Using the Polymerase Chain Reaction, Ramesh Ramakrishnan,
David J. Fink, and Myron Levine. HSV Vectors for Gene Therapy, David C.
Bloom. Investigation of the Anti-HSV Activity of Candidate Antiviral Agents,
Derrick J. Dargan. Index.