Mark A. Findeis and a panel of active researchers present their best methods not only for preparing, handling, and characterizing gene delivery agents, but also for gene delivery. To help those preparing and characterizing gene transfer agents, the contributors examine a broad range of compounds that bind with DNA to form the compact condensed phases that facilitate cellular delivery-among them peptide conjugates, synthetic polymers, and lipids. They also outline specific approaches to gene transfer in vivo, including direct delivery by intratumoral injection and indirect delivery by cell-specific targeting of DNA complexes, and discuss in detail many spectroscopic techniques for characterizing nonviral gene delivery agents.
Synthesis of Polyampholyte Comb-Type Copolymers Consisting of a Poly(l-lysine) Backbone and Hyaluronic Acid Side Chains for DNA Carrier
Atsushi Maruyama and Yoshiyuki Takei
Cationic _-Helical Peptides for Gene Delivery into Cells
Takuro Niidome and Haruhiko Aoyagi
Supramolecular Self-Assembly of Poly(ethylene glycol)-Block-Poly (l-lysine) Dendrimer with Plasmid DNA
Joon Sig Choi and Jong Sang Park
Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery
Gert W. Bos, Daan J. A. Crommelin, and Wim E. Hennink
Stabilization of Polycation-DNA Complexes by Surface Modification with Hydrophilic Polymers
David Oupicky, Martin L. Read, and Thierry Bettinger
Use of Disulfide Cationic Lipids in Plasmid DNA Delivery
Fuxing Tang and Jeffrey A. Hughes
Interactions of Lipid-Oligonucleotide Conjugates with Low-Density Lipoprotein
Erik T. Rump, Erik A. L. Biessen, Theo J. C. van Berkel, and Martin K. Bijsterbosch
Coupling of Nuclear Localization Signals to Plasmid DNA
Carole Neves, Daniel Scherman, and Pierre Wils
Progress Toward a Synthetic Virus: A Multicomponent System for Liver-Directed DNA Delivery
Bo-Hua Zhong, George Y. Wu, and Catherine H. Wu
Characterization of Polyampholyte Comb-Type Copolymer DNA Carriers
Yoshiyuki Takei, Atsushi Maruyama, Toshihiro Akaike, and Nobuhiro Sato
Methods for Studying the Formation of Polycation-DNA Complexes and Properties Useful for Gene Delivery
Martin L. Read, Thierry Bettinger, and David Oupicky
Characterization of DNA Condensates by Atomic Force Microscopy
Ye Fang and Jan H. Hoh
Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitting
Rona S. Scott and Jean-Michel H. Vos
Systemic Delivery of Therapeutic Proteins by Intramuscular Injection of Plasmid DNA
Holly M. Hortonand Suezanne E. Parker
Local Delivery of Therapeutic Proteins by Intratumoral Injection of Plasmid DNA-Lipid Complexes
Holly M. Horton and Suezanne E. Parker
Nonviral DNA Delivery from Polymeric Systems
Lonnie D. Shea and David J. Mooney
Promotion of Duplex and Triplex DNA Formation by Polycation Comb-Type Copolymers
Hidetaka Torigoe and Atsushi Maruyama
Lyophilization of Nonviral Gene Delivery Systems
S. Dean Allison and Thomas J. Anchordoquy
Ultraviolet Absorption and Circular Dichroism Spectroscopy of Nonviral Gene Delivery Complexes
Chad S. Braun, Lisa A. Kueltzo, and C. Russell Middaugh
Characterization of Synthetic Gene Delivery Vectors by Infrared Spectroscopy
Sirirat Choosakoonkriang, Christopher M. Wiethoff, Lisa A. Kueltzo, and C. Russell Middaugh
Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry
Brian A. Lobo, Sheila A. Rogers, Christopher M. Wiethoff, Sirirat Choosakoonkriang, Susan Bogdanowich-Knipp, and C. Russell Middaugh
Light-Scattering Techniques for Characterization of Synthetic Gene Therapy Vectors
Christopher M. Wiethoff and C. Russell Middaugh
Renal Gene Therapy
Yeong-Hau H. Lien and Li-Wen Lai