This collection of cutting-edge methodologies for studying membrane transporters and channels takes advantage of all the latest developments in biomedical research, including pharmacogenomcs, bioinformatics, and microarray technologies. The authors explain databases and tools for bioinformatics studies, provide practical guidelines for microarray experiments and data analysis, and illustrate the use of small angle X-ray scattering, nuclear magnetic resonance (NMR), and molecular modeling to study the structural biology of membrane transporters. Methods for exploring structure-function correlation, such as site-directed mutagenesis, immunocytochemistry, and confocal microcopy are also described, along with several that may help in the development of novel therapeutics.

Pharmacogenomics of Membrane Transporters: An Overview
Qing Yan

The IUBMB-Endorsed Transporter Classification System
Wolfgang Busch and Milton H. Saier, Jr.

Bioinformatics and Data Integration in Membrane Transporter Studies
Qing Yan

DNA Microarrays to Analyze Gene Expression in Animals With Altered Transporter Expression
Gary W. Miller

Microarray Data Analysis in Studies of Membrane Transporters
Donglei Hu

Laser Capture Microdissection to Examine Transporter Expression in Specific Cell Regions
Okkyung Rho and Gary W. Miller

Site-Directed Mutagenesis in the Study of Membrane Transporters
Renae M. Ryan, Ann D. Mitrovic, and Robert J. Vandenberg

Fluorescence Techniques for Studying Membrane Transport Proteins: The P-Glycoprotein Multidrug Transporter
Frances J. Sharom, Paula L. Russell, Qin Qu, and Peihua Lu

Reconstitution of Membrane Transporters
Frances J. Sharom and Paul D. W. Eckford

Equilibrium Binding and Transport Studies
Ana M. Ojeda, Dawn T. Bravo, Terry L. Hart, and Stanley M. Parsons

Electrophysiological Studies of Ion Channels and Therapeutic Potential with Adenovirus-Mediated Gene Transfer
Jihong Qu, Thai V. Pham, and Maria N. Obreztchikova

Antibody Production and Immunocytochemical Localization of Amino Acid Transporters
David V. Pow, Robert Sullivan, and Heather Scott

Xenopus laevis Oocytes
Stefan Bröer

Measurement of Intracellular pH
Frederick B. Loiselle and Joseph R. Casey

Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domains
Brian H. Shilton

Membrane Protein NMR Studies
Stanley J. Opella

Analysis of Soluble Sugar Permease Domains by Solution NMR
Thomas A. Wilkinson and Yuan Chen

Molecular Modeling and Molecular Dynamics Simulationsof Membrane Transporter Proteins
Donard S. Dwyer

Imaging Plasma Membrane Proteins by Confocal Microscopy
Stella W. Y. Tsang and Peter M. Haney

Index