A comprehensive collection of readily reproducible techniques for TNF production, characterization, mutagenesis, and detection in biological specimens. Also included are several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The collection offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of versatile, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.
Tumor Necrosis Factor as a Pharmacological Target
Pietro Ghezzi and Anthony Cerami
Production and Characterization of Recombinant Human and Murine TNF
Flavio Curnis and Angelo Corti
Purification of TNF Binding Proteins
Hartmut Engelmann, Dani Aderka, and David Wallach
Production and Characterization of Receptor-Specific TNF Muteins
Paul Ameloot and Peter Brouckaert
TNFa Promoter Polymorphysms
Silvia Fargion, Luca Valenti, Paola Dongiovanni, and Anna Ludovica Fracanzani
Detection of TNF and TNF Receptor mRNA in Cells and Tissues
Roberto Furlan, Giuliana Salani, Alessandra Bergami, and Gianvito Martino
Immunohistochemical Localization of TNFa and Its Receptors in the Rodent Central Nervous System
Paolo Bigini and Tiziana Mennini
TNF Signaling: Key Protocols
Marta Muzio and Simona Saccani
More Than One Way to Die: Methods to Determine TNF-Induced Apoptosis and Necrosis
Tom Vanden Berghe, Geertrui Denecker, Greet Brouckaert, Dmitri Vadimovisch Krysko, Katharina D'Herde, and Peter Vandenabeele
Assessment of TNFa-Induced Endothelial Damage Through the Loss of Its Barrier Function
Elisabetta Ferrero
Genetic Engineering in the Mouse: Tuning TNF/TNFR Expression
Eleni Douni, Maria Alexiou, and George Kollias
TNFa in Experimental Diseases of the CNS
Roberto Furlan, Pia Villa, Giorgio Senaldi, and Gianvito Martino
In Vivo Vascular Leakage Assay
Maria Elena Ferrero
Animal Models of Endotoxic Shock
Pia Villa and Pietro Ghezzi
Collagen-Induced Arthritis as a Model for Rheumatoid Arthritis
Richard O. Williams
Myocardial Infarction: Animal Models
Stefano Chimenti, Eleonora Carlo, Serge Masson, Antonio Bai, and Roberto Latini
Tumor Vascular Therapy With TNF: Critical Review on Animal Models
Timo L. M. ten Hagen and Alexander M.M. Eggermont
Strategies for Improving the Anti-Neoplastic Activity of TNF by Tumor Targeting
Angelo Corti
Index