The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.

Treatment of Tissue Sections for In Situ Hybridization
Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson

Preparation of Template DNA and Labeling Techniques
Peter J. Roche

In Situ Hybridization Using cRNA Probes: Isotopic and Nonisotopic Detection Methods
Ian A. Darby, Teresa Bisucci, Alexis Desmoulière, and Tim D. Hewitson

Tyramide Signal Amplification for DNA and mRNA In Situ Hybridization
Ernst J. M. Speel, Anton H. N. Hopman, and Paul Komminoth

Expression Analysis of Murine Genes Using In Situ Hybridization With Radioactive and Nonradioactively Labeled RNA Probes
Anne Chotteau-Lelièvre, Pascal Dollé, and Françoise Gofflot

Nonradioactive In Situ Hybridization on Frozen Sections and Whole Mounts
Julia Asp, Alexandra Abramsson, and Christer Betsholtz.

In Situ Hybridization of Whole-Mount Embryos
Murray Hargrave, Josephine Bowles, and Peter Koopman

In Situ Detection of Epstein-Barr Virus and Phenotype Determination of EBV-Infected Cells
Gerald Niedobitek and Hermann Herbst

PNA-In Situ Hybridization Method for Detection of HIV-1 DNA in Virus-Infected Cells and Subsequent Detection of Cellular and Viral Proteins
Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda

Electron Microscopy and In Situ Hybridization: Expression of P2Y2 Receptor mRNA in the Cerebellum
Andrzej Loesch and Rainer Glass

In Situ Hybridization Using Riboprobes on Free-Floating Brain Sections
Neil C. Owens, F. Martin Hess, and Emilio Badoer

An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells
Changsun Choi, Laurie G. Hudson, Pierre Savagner, and Donna F. Kusewitt

Identification of Transplanted Human Cells in Animal Tissues
Daniel Benten, Kang Cheng, and Sanjeev Gupta

In Situ Hybridization to Plant Tissues and Chromosomes
AndreasHouben, Sharon J. Orford, and Jeremy N. Timmis

Histochemical Localization of Cell Proliferation Using In Situ Hybridization for Histone mRNA
Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby

Histochemical Localization of Apoptosis With In Situ Labeling of Fragmented DNA
Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby

The Use of Combined Immunohistochemical Labeling and In Situ Hybridization to Colocalize mRNA and Protein in Tissue Sections
Malcolm D. Smith, Michael Ahern, and Mark Coleman

Semiquantitative In Situ Hybridization Using Radioactive Probes to Study Gene Expression in Motoneuron Populations
Paul D. Storer and Tracey DeLucia

Quantitative In Situ Hybridization of Tissue Microarrays
Adrian M. Jubb, Thinh Q. Pham, Gretchen D. Frantz, Franklin V. Peale, Jr., and Kenneth J. Hillan

Index