Leading clinicians and scientists in solid organ transplantation review the current status of the field and describe cutting-edge techniques for detecting the immune response to the allografted organ. The authors present the latest techniques for HLA typing, detecting HLA antibodies, and monitoring T-cell response, and examine more specialized methods utilizing proteomics, laser dissection microscopy, and real-time polymerase chain reaction. The area of tolerance induction and reprogramming of the immune system is also covered, along with a discussion of up-to-date methods of organ preservation, of today's optimal immunosuppressive drug regimens, as well as the difficulty of mimicking chronic rejection in experimental models. Introductory chapters provide a theoretical update on current practices in renal, liver, islet, and lung transplantation and on the pathways of antigen presentation and chronic rejection.

Current Status of Renal Transplantation
Christopher J. Callaghan and J. Andrew Bradley

Current Status of Liver Transplantation
Peter J. Friend and Charles J. Imber

Current Status of Clinical Islet Cell Transplantation
Jonathan R. T. Lakey, Mohammadreza Mirbolooki, and A. M. James Shapiro

Current Status of Lung Transplantation
Allan R. Glanville

Chronic Rejection in the Heart
Philip Hornick and Marlene Rose

Direct and Indirect Allorecognition
Philip Hornick

HLA Typing and Its Influence on Organ Transplantation
Stephen Sheldon and Kay Poulton

Strategies for Gene Transfer to Solid Organs: Viral Vectors
Charlotte Lawson

Nonviral Vectors
Louise Collins

Detection and Clinical Relevance of Antibodies After Transplantation
John D. Smith and Marlene Rose

Reprogramming the Immune System Using Antibodies
Luis Graca and Herman Waldmann

In Vitro Assays for Immune Monitoring in Transplantation
Maria P. Hernandez-Fuentes and Alan Salama

Proteomics and Laser Microdissection
Emma McGregor and Ayesha De Souza

Real-Time Quantitative Polymerase Chain Reaction in Cardiac Transplant Research
Leanne E. Felkin, Anne B. Taegtmeyer, and Paul J. R. Barton

Organ Preservation
Mark Hicks, Alfred Hing, Ling Gao, Jonathon Ryan, and Peter S. MacDonald

Pharmacological Manipulation of the Rejection Response
Peter Mark Anthony Hopkins

Experimental Models of Graft Arteriosclerosis
Bezhad Soleimani and Victor C. Shi

Index