This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.

Table of Contents

Preface¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿...Robert J. McMahon
Preparation of avidin conjugates¿¿¿¿¿¿¿¿¿ Rosaria Haugland and Mahesh Bhalgat
Coupling of antibodies with biotin¿¿¿¿¿¿¿¿¿¿...Rosaria Haugland and Wendy You
Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and streptavidin¿¿¿¿¿¿¿¿.Sara R. Zwart and Brandon J. Lewis
Use of the Avidin (Imino)biotin System as a general approach to affinity precipitation¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿..Ruth Freitag and Frank Hilbrig
Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis.........................................................................................Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed Salman Lateef, and Shalini Gupta
Functionality Screening of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿..Nicolas Humbert and Thomas R. Ward
Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿..Andreas Ebner, Markus Marek, Karl Kaiser, Gerald Kada, Christoph D. Hahn, Bernd Lackner, and Hermann J. Gruber
Quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins¿¿¿¿¿¿¿¿¿¿..Christoph Rosli, Jascha-N. Rybak, Dario Neri, and Giuliano Elia
High yield production and purification of recombinant T7-tag-mature-streptavidin in glucose stressed E. coli¿.Nicolas Humbert, Peter Schurmann, Andrea Zocchi, Jean-Marc Neuhaus and Thomas R. Ward
Endogenous biotin in rat brain: implications for false-positive resultswith avidin- and streptavidin-biotin techniques..Bruce E. McKay, Michael L. Molineux, and Ray W. Turner
Pseudo-Immunolabelling with the Avidin-Biotin-Peroxidase Complex (ABC) due to the presence of Endogenous Biotin in the Retinä..Willem Kamphuis and Jan Klooster
Use of synthetic peptides for identifying biotinylation sites in human histones¿¿¿¿Gabriela Camporeale, Yap Ching Chew, Alice Keuh, Gautam Sarath and Janos Zempleni
Detection and quantitation of DNA methylation using a biotin/avidin microplate assay¿Kirsten Liebert and Albert Jeltsch
Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin¿¿¿¿¿¿¿¿¿¿¿Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux
Mammalian lectin as a tool in glycol- and histochemistry with relevance for diagnostic procedure¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿¿Karel Smetana, Jr. and Sabine Andre
A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin¿¿¿¿¿¿¿¿¿¿¿ Anna Bogusiewicz