Bültmann & Gerriets
DNA and RNA Isolation Techniques for Non-Experts
von Akash Gautam
Verlag: Springer International Publishing
Reihe: Techniques in Life Science and Biomedicine for the Non-Expert
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ISBN: 978-3-030-94230-4
Auflage: 1st ed. 2022
Erschienen am 29.03.2022
Sprache: Englisch
Umfang: 203 Seiten

Preis: 90,94 €

Inhaltsverzeichnis
Biografische Anmerkung

1-Basic idea about nucleic acids for non-experts



2-General steps during isolation of DNA and RNA



3-Phenol-chloroform DNA isolation method



4-RNA isolation by the guanidinium-acid-phenol method



5-Spin column-based isolation of nucleic acid



6-Isolation of plasmid DNA by alkaline lysis



7-DNA Isolation by Hydrophilic Ionic Liquid Treatment



8-Lithium Chloride based isolation of DNA and RNA



9-Isolation of Bacteriophage DNA by PEG Method



10-DNA isolation by Chelex Method



11-DNA isolation by Lysozyme and Proteinase K



12-Salting-out method for blood samples



13-CTAB or SDS based isolation of plant's DNA



14-DNA extraction by spooling method



15-Magnetic bead-based nucleic acid isolation



16-Density gradient-based nucleic acid isolation



17-DNA extraction from agarose gel though paper-strips



18-TRANSFORMATION OR GENETIC MODIFICATION OF CELLS/ORGANISMS



19-GENE CLONING AND VECTORS



20-POLYMERASE CHAIN REACTION (PCR)



21-Southern and Northern Blotting



22-GENOME MAPPING



23-Applications of DNA Sequencing Technologies for Current Research



Dr. Akash Gautam is an Assistant Professor at the Center for Neural and Cognitive Sciences, University of Hyderabad. Dr. Gautam is currently serving as a section editor for the Encyclopedia of Animal Cognition and Behavior (Springer Nature c. 2020).


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