This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

Nayana Patil, PhD

Associate Professor, School of Bioengineering Sciences and Research, MIT ADT University

Aruna Sivaram, PhD

Associate Professor, School of Bioengineering Sciences and Research, MIT ADT University

1.The Recombinant DNA technology era.- 2.Vectors: Guide to gene delivery vehicles.- 3.Gene isolation methods: beginner's guide.- 4.Restriction Enzymes-Tools for DNA cloning.- 5.Overview of Gene cloning strategies.- 6.Fundamental techniques of recombinant DNA transfer.- 7.Selection, screening and analysis of recombinant clones.- 8.Recent trends and advances.- 9.Applications of Recombinant DNA technology.- 10.Ethical and safety concerns of recombinant DNA Technology.