The 12th Origeniana conference took place at the Hebrew University of Jerusalem, Israel, in June 2017. The theme of the conference was Origen's Legacy in the Holy Land - A Tale of Three Cities: Jerusalem, Caesarea and Bethlehem. The present volume contains a large selection from the papers that were given at the conference. Many of the papers discuss various aspects and new perspectives of Origen's career and legacy in the Holy Land in the broad sense. As was the case also in previous conferences, there was an opportunity to deliver papers on other aspects of current Origen research. The volume contains five sections: Jerusalem and the Holy Land: Historical and Mystical Geography; The School of Caesarea and Eusebius; Origen's Latin Legacy: Rufinus and Jerome; Tradition, Innovation, and Heritage: Origen's Exegesis and Theology; and The Origenist Legacy: From Evagrius to Balthasar.