Preface
About the Authors
Section I: The Biology behind the Technology
Chapter 1: Fundamental Technologies
Molecular Cloning
Genomic Libraries
Amplification of DNA Using PCR
DNA Sequencing Technologies
Sequencing Whole Genomes
Genomics
Chapter 2: Fundamental Concepts in Immunology
The Immune Response
Functions of the Immune System
Cells of the Immune System: Cell-Mediated
Tissues of the Immune System
Humoral Immunity
Types of Antibodies: Applications
Immunological Techniques
Chapter 3: The Genetic Basis of Disease
Chromosomal Disorders and Gene Mapping
Single-Gene Disorders
Polygenic Disorders and Gene Clustering
Mitochondrial Disorders
Chapter 4: Immune Pathogenesis
Models of Immune System Lesions
Inflammation and Immune Hypersensitivity
Immunodeficiency Disorders and Defects in Development of the Immune System
Chapter 5: Microbial Pathogenesis
Introduction
Bacterial Infections
Viral Infections
Section II: Production of Therapeutic Agents
Chapter 6: Modulation of Gene Expression
Manipulating Gene Expression in Prokaryotes
Heterologous Protein Production in Eukaryotic Cells
Directed Mutagenesis
Chapter 7: Genetic Engineering of Plants
Plant Transformation with the Ti Plasmid of A. tumefaciens
Physical Transfer of Genes to Plants
Chloroplast Engineering
Transient Gene Expression
Molecular Pharming
Edible Vaccines
Section III: Diagnosing and Treating Human Disease
Chapter 8: Molecular Diagnostics
Immunological Approaches to Detect Protein Biomarkers of Disease
DNA-Based Approaches to Disease Diagnosis
Detecting RNA Signatures of Disease
Chapter 9: Protein Therapeutics
Pharmaceuticals
Recombinant Antibodies
Enzymes
Lactic Acid Bacteria
Chapter 10: Nucleic Acid Therapeutic Agents and Human Gene Therapy
Treating Genetic and Nongenetic Disorders
Targeting Specific mRNAs and DNAs
Viral Delivery Systems
Nonviral Nucleic Acid Delivery Systems
Gene Therapy
Chapter 11: Vaccines
Vaccination: Overview
Subunit Vaccines
Peptide Vaccines
Dendritic Cell Vaccines
DNA Vaccines
Attenuated Vaccines
Vector Vaccines
Adjuvants
Systems Biology and Evaluation of Vaccines
Chapter 12: Societal Issues
Safety and Ethical Issues
Patenting Biotechnology
Economic Issues
Glossary
Index