Marco A. van den Berg, PhD

Principal Scientist Applied Biochemistry and Screening

DSM Biotechnology Center

Delft, The Netherlands

 Karunakaran Maruthachalam, PhD

Current: Research Scientist

Global Marker Technology Lab, DuPont-Pioneer

DuPont Knowledge Center

Andhra Pradesh, India

 Previous: Molecular Biologist

California Seed and Plant Labs

Pleasant Grove, CA, USA

Preface

Contributors

I.                   Introduction

1.      Fungal Transformation: From Protoplasts to Targeted Recombination Systems

Juan F. Martín

II.                Transformation Methods: Protoplast Transformation

2.      Protoplast Transformation for Genome Manipulation in Fungi

Aroa Rodriguez-Iglesias and Monika Schmoll

3.      Trichoderma Transformation Methods

Mónica G. Malmierca, Rosa E. Cardoza and Santiago Gutiérrez

4.      Transformation of Mucor circinelloides f. lusitanicus Protoplasts

Victoriano Garre, José Luis Barredo and Enrique A. Iturriaga

5.      Transformation of Saccharomyces cerevisiae: Spheroplast Method

Shigeyuki Kawai and Kousaku Murata

III.             Transformation Methods: Electroporation

6.      Electroporation Mediated DNA Transformation of Filamentous Fungi

B.N. Chakraborty

7.      Chemical Transformation of Candida albicans

Sophie Bachellier-Bassi and Christophe d'Enfert

8.      Electroporation of Pichia pastoris

Knut Madden, Ilya Tolstorukov and James Cregg

9.      Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli)

Kostyantyn Dmytruk and Andriy Sibirny

IV.             Transformation Methods: Particle Bombardment

10.  Biolistic Transformation for Delivering DNA into the Mitochondria

11.  Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome Transformants

Jingwen Zhou, Liming Liu, Guocheng Du and Jian Chen

12.  Use of the Biolistic Particle Delivery System to Transform Fungal Genomes

V. S. Junior Te'o and K. M. Helena Nevalainen

13.  Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment

Eiji Sakuradani, Hiroshi Kikukawa, Seiki Takeno, Akinori Ando, Sakayu Shimizu and Jun Ogawa

V.              Transformation Methods: Agrobacterium-Mediated Transformation

14.  Agrobacterium tumefaciens-Mediated Transformation

Rasmus John Normand Frandsen

15.  Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red Yeasts

Giuseppe Ianiri and Alexander Idnurm

16.  Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporum

Manish Pareek, Mahak Sachdev, Meenakshi Tetorya and Manchikatla V. Rajam

VI.             Transformation Methods: Li-acetate Transformation

17.  High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method

R. Daniel Gietz

18.  Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying Mechanism

Shigeyuki Kawai and Kousaku Murata

19.  Transformation of Lithium Acetate-treated Neurospora crassa

John V. Paietta

VII.          Transformation Methods: New developments

20.  Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast Cells

Yevhen Filyak, Nataliya Finiuk, Nataliya Mitina, Alexander Zaichenko and Rostyslav Stoika

21.  Transformation of Fungi Using Shock Waves

Miguel A. Gómez-Lim, Denis Magaña Ortíz, Francisco Fernández and Achim M. Loske

VIII.       Exogenous DNA: Uptake of DNA

22.  Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast Genetics

Petar Tomev Mitrikeski

23.  Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell Cycle

Aurelijus Zimkus, Audrius Misiunas, Arunas Ramanavicius and Larisa Chaustova

IX.             Exogenous DNA: Integration of DNA

24.  Recombination and Gene Targeting in Neurospora

Keiichiro Suzuki and Hirokazu Inoue

25.  Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker Approach

Mark Arentshorst, Jing Niu and Arthur F.J. Ram

26.  REMI in Molecular Fungal Biology

Aurin M. Vos, Luis G. Lugones and Han A. B. Wösten

27.  TALEN-Based Genome Editing in Yeast

Ting Li, David A. Wright, Martin H. Spalding and Bing Yang

Index.

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ¿

Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.