Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ¿
Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.

PrefaceContributorsI.                   Introduction1.      Fungal Transformation: From Protoplasts to Targeted Recombination SystemsJuan F. MartínII.                Transformation Methods: Protoplast Transformation2.      Protoplast Transformation for Genome Manipulation in FungiAroa Rodriguez-Iglesias and Monika Schmoll3.      Trichoderma Transformation MethodsMónica G. Malmierca, Rosa E. Cardoza and Santiago Gutiérrez4.      Transformation of Mucor circinelloides f. lusitanicus Protoplasts Victoriano Garre, José Luis Barredo and Enrique A. Iturriaga5.      Transformation of Saccharomyces cerevisiae: Spheroplast MethodShigeyuki Kawai and Kousaku MurataIII.             Transformation Methods: Electroporation6.      Electroporation Mediated DNA Transformation of Filamentous FungiB.N. Chakraborty7.      Chemical Transformation of Candida albicans Sophie Bachellier-Bassi and Christophe d¿Enfert8.      Electroporation of Pichia pastoris Knut Madden, Ilya Tolstorukov and James Cregg9.      Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareli) Kostyantyn Dmytruk and Andriy SibirnyIV.             Transformation Methods: Particle Bombardment10.  Biolistic Transformation for Delivering DNA into the MitochondriaArianna Montanari, Monique Bolotin-Fukuhara, Mario Fazzi D¿Orsi, Cristina De Luca, Michele M. Bianchi and Silvia Francisci11.  Bolistic Transformation of Candida glabrata for Homoplasmic Mitochondrial Genome TransformantsJingwen Zhou, Liming Liu, Guocheng Du and Jian Chen12.  Use of the Biolistic Particle Delivery System to Transform Fungal GenomesV. S. Junior Te¿o and K. M. Helena Nevalainen13.  Transformation of Zygomycete Mortierella alpina Using Biolistic Particle BombardmentEiji Sakuradani, Hiroshi Kikukawa, Seiki Takeno, Akinori Ando, Sakayu Shimizu and Jun OgawaV.              Transformation Methods: Agrobacterium-Mediated Transformation14.  Agrobacterium tumefaciens-Mediated Transformation Rasmus John Normand Frandsen15.  Agrobacterium tumefaciens-Mediated Transformation of Pucciniomycotina Red YeastsGiuseppe Ianiri and Alexander Idnurm16.  Glass-bead and Agrobacterium-mediated Genetic Transformation of Fusarium oxysporumManish Pareek, Mahak Sachdev, Meenakshi Tetorya and Manchikatla V. RajamVI.             Transformation Methods: Li-acetate Transformation17.  High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG MethodR. Daniel Gietz18.  Transformation of Intact Cells of Saccharomyces cerevisiae: Lithium Methods and Possible Underlying MechanismShigeyuki Kawai and Kousaku Murata19.  Transformation of Lithium Acetate-treated Neurospora crassaJohn V. PaiettaVII.          Transformation Methods: New developments20.  Application of Novel Polymeric Carrier of Plasmid DNA for Transformation of Yeast CellsYevhen Filyak, Nataliya Finiuk, Nataliya Mitina, Alexander Zaichenko and Rostyslav Stoika21.  Transformation of Fungi Using Shock WavesMiguel A. Gómez-Lim, Denis Magaña Ortíz, Francisco Fernández and Achim M. LoskeVIII.       Exogenous DNA: Uptake of DNA22.  Pathways and Mechanisms of Yeast Competence: A New Frontier of Yeast GeneticsPetar Tomev Mitrikeski23.  Evaluation of Competence Phenomenon of Yeast Saccharomyces cerevisiae by Lipophilic Cations Accumulation and FT-IR Spectroscopy. Relation of Competence to Cell CycleAurelijus Zimkus, Audrius Misi¿nas, Ar¿nas Ramanavi¿ius and Larisa ChaustovaIX.             Exogenous DNA: Integration of DNA24.  Recombination and Gene Targeting in NeurosporaKeiichiro Suzuki and Hirokazu Inoue25.  Efficient Generation of Aspergillus niger Knock Out strains by Combining NHEJ Mutants and a Split Marker ApproachMark Arentshorst, Jing Niu and Arthur F.J. Ram26.  REMI in Molecular Fungal BiologyAurin M. Vos, Luis G. Lugones and Han A. B. Wösten27.  TALEN-Based Genome Editing in YeastTing Li, David A. Wright, Martin H. Spalding and Bing YangIndex.

Marco A. van den Berg, PhD

Principal Scientist Applied Biochemistry and Screening

DSM Biotechnology Center

Delft, The Netherlands

 Karunakaran Maruthachalam, PhD

Current: Research Scientist

Global Marker Technology Lab, DuPont-Pioneer

DuPont Knowledge Center

Andhra Pradesh, India

 Previous: Molecular Biologist

California Seed and Plant Labs

Pleasant Grove, CA, USA